You Receive a 1.5 mL vial of a 12.5 kDa protein that has a concentration of...

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Chemistry

You Receive a 1.5 mL vial of a 12.5 kDa protein that has aconcentration of 1.45 mM. When you test its OD280 in a 1cm cuvette,it is 8.434.

How much total protein, in mg, do you have?

A) 0.027 B) 27.2 C) 27187 D) 15.8 E) 158137

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3.7 Ratings (620 Votes)

given that 1.5 mL of 1.45mM concentration protein received.

Let us convert this into number of millimoles as follows.

1.5 mL of 1.45 mM protein = 1.5 mL x 1.45 millimole / 1000 mL

                                       = 1.5 x 1.45/1000 millimole

                                       = 0.002175 millimole

       given that the protein has the molar mass 12.5 kDa

                                                                = 12500 Da

                                                                = 12500 g / mol

                                                               = 12500 mg / mmol

Therefore 0.002175 mmole protein has the mass = 0.002175 mmol x 12500 mg/mmol

                                                                  = 27.1875 mg

                                                                  = 27.2 mg

Hence the option (B) is the Answer.


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