You are planning to do some experiments to demonstrate the dynamic nature of microtubules. How you...

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Biology

You are planning to do some experiments to demonstrate thedynamic nature of microtubules. How you would fluorescently labelmicrotubules in order to use for FRAP experiments inside acell?
Once you have labeled microtubules, you decide to treat thecells with taxol and then perform FRAP What would be your controlfor this experiment? What are your predictions for the experimentaland control experiment results?

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Ans The quantification of the immobile fraction of molecules is only possible by FRAP This parameter is of great interest to avoid epiphenomenal observations by svFCS While the two methods are inversely sensitive to the concentration of fluorescent probes there exists a range for which measurements are compatible Implementing FRAP and FCS on the same setup is easy to do with an acoustooptic modulator AOM to precisely adjust the laser power A small region of a surface or volume containing mobile fluorescent molecules or See Answer

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