)Tabulate how transmission electron microscopy (TEM) differs from traditional light microscopy regarding (a) sample preparation (4), (b)...

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Biology

)Tabulate how transmission electron microscopy (TEM) differsfrom traditional light microscopy regarding (a) sample preparation(4), (b) processing (4), (c) instrumentation (4), (d) detection (4)and (e) the structures that can be observed (3), (f) staining (3),(g) IHC (4) and (h) ISH (4).

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Transmission electron microscopy TEM Light Microscope Transmission electron microscopy TEM is a microscopy technique in which a beam of electrons is transmitted through a specimen to form an image The specimen is most often an ultrathin section less than 100 nm thick or a suspension on a grid A light microscope LM is an instrument that uses visible light and magnifying lenses to examine small objects not visible to the naked eye or in finer detail than the naked eye allows Sample Preparation For TEM samples must be cut into very thin crosssections This is to allow electrons to pass right through the sample Sample Preparation Preparation often involves nothing more than mounting a small piece of the specimen in a suitable See Answer

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