What is Coomassie blue? How does it function? (1.5 marks) As an alternative to coomassie blue staining,...

90.2K

Verified Solution

Question

Biology

What is Coomassie blue? How does it function?
(1.5 marks)
As an alternative to coomassie blue staining, a silver nitratesolution can be used. How does silver nitrate
staining differ and under which situations might it be used?
In which ways are vertical polyacrylamide gels and horizontalagarose gels the same? In which ways do
they differ?
5
In which loaded fraction (lane) would you expect to find themost proteins? The least? Explain
6
In your own words, provide a brief overview of next weekâ€™s lab.Include the experi

Answer & Explanation Solved by verified expert

3.9 Ratings (395 Votes)

Coomassie Brilliant blue CBB is a stain that is used in PAGE gels The sulfonic acid group of the stain ofrms ionic bonds with the amino group of proteins Apart from this there is also weak vander waals interaction between the dye and proteins The sensitivity of the detection of proteins in polyacrylamide gels by CBB depends upon the type of dye used CBB R250 can detect about 100 ng protein whereas CBB G250 is much more sensitive as it would detect See Answer

Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:

Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!

Become a Member