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You run an ion exchange column, eluting your protein with a high NaCl buffer. Would you...

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Chemistry

You run an ion exchange column, eluting your protein with a highNaCl buffer. Would you be able to run a size exclusion columnimmediately after the IEX column or would you have to dialyze yoursample to change the buffer first?

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3.9 Ratings (531 Votes)
Dear friend the answer is yes you would be able to run a size exclusion column immediately after the IEX column because A small amount of salt will help prevent nonspecific interactions between the protein and stationary phase This will allow all protein molecules to run consistently over the length of the    See Answer
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