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What are the step to do electrophorese 2D in order to purify the protein after cloning...

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Biology

What are the step to do electrophorese 2D in order to purify theprotein after cloning ?

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2D gel electrophoresis consists of isoelectric focussing with SDS polyacrylamide gel electrophoresis This separates proteins based on isoelectric point The isoelectric point is the pH at which protein has no net charge This is done with an immobilized pH gradient gel strip or with    See Answer
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