Steps a) add 20 grams of oysters to 20 mL of saline to give a 1:2 dilution...

60.1K

Verified Solution

Question

Biology

Steps
a) add 20 grams of oysters to 20 mL of saline to give a 1:2dilution slurry
b) Transfer 5 grams of the slurry into a conical tube.
c) Prepare a 1:10 serial dilution by adding 20mL of saline tothe measured 5 g of slurry ( this step effects a 1:5 dilution butthat sample was already diluted 1:2)
d) Prepare a 10^-1, 10^-2, and 10^-3 serial dilution in culturetubes with 9 mL of saline
e) plate 0.1 ml of each dilution onto an agar plate.
f) two plates from the 10^-3 dilution made colonies of 112colonies and 58 colonies.
1. Show calculations for the serial dilutions with units
2. calculate the CFU/g for each plate with colonies withunits

Answer & Explanation Solved by verified expert

4.3 Ratings (790 Votes)

Note the following points beforestarting Note that using this method theanswer in CFUs per one milliliter or per one gram is derivedAnswers may need to be adjusted if the number of CFUs per sampleother than a milliliter or gram is requested Assume 1gram 1 ml 1 ml of water does indeed weigh 1 gram That isactually how the ml is defined Use only those plates with colonycounts between 25 and 300 With duplicate or triplicateplating from the same dilution take the average of the See Answer

Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:

Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!

Become a Member