1. Outline two experimental methods to test the hypothesis that marijuana alters a) gene expression and...

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Biology

1. Outline two experimental methods to test the hypothesis thatmarijuana alters a) gene expression and b) protein expression inmice brains. Explain also how you will know the specific identityof the genes and proteins that are altered. You will need to searchthe literature for biochemical techniques that would beappropriate.
2. Assume you find a gene that is upregulated in mice brains inresponse to marijuana. How would you determine exactly where in themouse brain this gene is expressed and to what extent? In addition,how would you determine the subcellular localization of the proteincorresponding to this gene? Briefly, outline five experiments toaddress these questions. You will need to search the literature forbiochemical techniques that would be appropriate.

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1 Outline two experimental methods to test the hypothesis that marijuana alters a gene expression and b protein expression in mice brains Explain also how you will know the specific identity of the genes and proteins that are altered You will need to search the literature for biochemical techniques that would be appropriate a For analysis of gene expression the most widely used technique is RTqPCR Reverse Transcription followed by Real Time PCR In order to check if in a given tissue sample the transcription status of a set of genes is altered we will follow the following protocol Mouse will be used as model organism lets say we have taken 12 mice in total These mice will be divided into 4 groups group A B C and D thus there will be 3 mice in each group labeled as 1 2 and 3 Group A mice will be fed with marijuana 1 gm per day Group B mice will be fed with marijuana 2 gm per day Group C mice will be fed with marijuana 4 gm per day Group D mice will not be fed with marijuana and normal diet will be provided to them Except for the marijuana intake rest all the conditions in which See Answer

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