Question 1) Microscopy. Give realistic examples for (i) standard light microscopy, (ii) immunofluorescence microscopy, and (iii) electron...

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Biology

Question 1) Microscopy. Give realistic examples for (i) standardlight microscopy, (ii)
immunofluorescence microscopy, and (iii) electron microscopy onhow these three distinct forms of
microscopy can be used to examine biological questions in celland molecular biology. Indicate
limitations for these techniques, including if living cells ortissue or fixed (dead) samples are involved,
what resolution is reasonably possible (smallest object that canbe seen) and if special reagents such as
antibodies are needed.

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Standard light microscopy Schleiden and Schwann used the standerd light microscopeto describe individual cells as the fundamental unit of life The microscope has several lenses which are used to magnify the image of a specimen under study The total magnification is a product of the magnification of the individual lenses which is if the objective lens magnifies 100fold along with the eyepiece which helps magnify 10fold thus the final magnification recorded by the humans or on film will be See Answer

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