Problem 1. Mitochondria may be prepared from cells and tested incell free experiments. If supplied with pyruvate and oxygen,isolated mitochondria are capable of aerobic metabolism.
C. You as the investigator measure the energy state of themitochondria as the ratio [NADH]/[NAD+]
1. Describe how the ratio is high and low for high and lowenergy charge, respectively
2. You remove the supply of oxygen. Explain how the ratiochanges in response
3. You add an inhibitor of ATP synthase. Explain how the ratiochanges in response
D. You as the investigator makes three measurements at the endof a test that lasts 10 min (exact time is not important). Youmeasure 10 nmol (10-9 moles) of pyruvate consumed (this number isimportant). You also measure the nmol of CO2 and citrateproduced.
1. Explain how 30 nmol of CO2 were produced.
2. Explain how 0 nmol of citrate were produced.
3. Based on the measurements above, predict how many nmol ofacetyl-CoA were produced. Explain.
