OPEN QUESTIONS: You are researching mechanisms involved in wdf1 gene expression regulation in yeast 1. Please shortly...

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Biology

OPEN QUESTIONS: You are researching mechanisms involved in wdf1gene expression regulation in yeast
1. Please shortly describe how to identify enhancers regulatingexpression of the wdf1 gene.
2. You have discovered that Wou1 protein is the transcriptionalfactor that is involved in wdf1 activation. You next goal is toidentify other factors, that are interacting with Wou1 protein andare required for wdf1 activation. Please shortly describeexperimental approaches to achieve this goal.
3. You have discovered that Wou1 protein is the transcriptionalfactor that is involved in wdf1 activation. You next goal is toidentify other factors, that are interacting with Wou1 protein andare required for wdf1 activation. Please shortly describealternative experimental approaches to achieve this goal.

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3.6 Ratings (345 Votes)

1 Identification of enhancers Enhancers can be identified by a method called the Enhancer trap In enhancer trap a reporter gene say GFP is fused to a minimal promoter that contains the core promoter elements like TATA box and transcription start site Using a Pelement transposon See Answer

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