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Molecular weight: 93910.16 Theoretical pI: 6.03 1. 1. Based on the pI of Taq, what pH...

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Chemistry

Molecular weight: 93910.16 Theoretical pI: 6.03 1.

1. Based on the pI of Taq, what pH buffer would you have to useto get Taq polymerase to stick to an anion exchanger?

Briefly discuss the advantages and disadvantages of using a saltor pH in eluting proteins from an ion-exchange column.

Please place details explaining how you arrived to ech answerfor question 1

Answer & Explanation Solved by verified expert
4.2 Ratings (640 Votes)
Given Taq protein Molecular weight 9391016 Theoretical pI 603 When the pH is lower than the pI the amino acid will carry a net positive charge When the pH is higher than pI then the amino acid carries a net negative charge We need to use anion exchanger therefore molecule must be    See Answer
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