METABOLISM (FERMENTATION) What are the expected results/did you get them. Tube A B C Initial Reading Final Reading Volume of the gas bubble created If not...

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Biology

METABOLISM(FERMENTATION) What are the expected results/did you getthem.
Tube A B C Initial Reading Final Reading Volume of the gas bubble created If not why and Explain
1 5 ml phosphate buffer 1 5 ml phosphate buffer 5 ml yeast 1mL 1.5mL 0mL
2 5 ml phosphate buffer 5 ml glucose 5 ml yeast 1.5mL 6.5mL 5mL
3 5 ml of 0.1 M MgSO4 5 ml glucose 5 ml yeast 1mL 8mL 7mL
4 5 ml of 50 mM EDTA 5 ml glucose 5 ml yeast 1mL 6mL 5mL
5 5 ml of 100 mM EDTA 5 ml glucose 5 ml yeast 1.5mL 2.5mL 1mL
6 5 ml of 250 mM EDTA 5 ml glucose 5 ml yeast 1mL 1mL 0mL
7 5 ml pyruvate/EDTA 5 ml glucose 5 ml yeast 1mL 8.5mL 7.5mL
8 5 ml phosphate buffer 5 ml of 0.1 M lactose 5 ml yeast 1mL 2mL 1mL
9 5 ml phosphate buffer 5 ml of 0.1 M galactose 5 ml yeast 1.5mL 2mL 0.5mL
Complete the last coloumn and state why these reactionsoccured. For example, in tube 3, there was a larger gas bubblebecause there was a presence of magnesium ions which meant theenzyme elonase was able to function.

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4.2 Ratings (515 Votes)

Fermentation pathway in yeast converts glucose to CO2 which is measured as gas bubble formation in the tubes In the 1st tube only phosphate buffer is there no carbohydrates Hence this must be a control See Answer

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METABOLISM(FERMENTATION)	What are the expected results/did you getthem.
Tube	A	B	C	Initial Reading	Final Reading	Volume of the gas bubble created	If not why and Explain
1	5 ml phosphate buffer	1 5 ml phosphate buffer	5 ml yeast	1mL	1.5mL	0mL
2	5 ml phosphate buffer	5 ml glucose	5 ml yeast	1.5mL	6.5mL	5mL
3	5 ml of 0.1 M MgSO4	5 ml glucose	5 ml yeast	1mL	8mL	7mL
4	5 ml of 50 mM EDTA	5 ml glucose	5 ml yeast	1mL	6mL	5mL
5	5 ml of 100 mM EDTA	5 ml glucose	5 ml yeast	1.5mL	2.5mL	1mL
6	5 ml of 250 mM EDTA	5 ml glucose	5 ml yeast	1mL	1mL	0mL
7	5 ml pyruvate/EDTA	5 ml glucose	5 ml yeast	1mL	8.5mL	7.5mL
8	5 ml phosphate buffer	5 ml of 0.1 M lactose	5 ml yeast	1mL	2mL	1mL
9	5 ml phosphate buffer	5 ml of 0.1 M galactose	5 ml yeast	1.5mL	2mL	0.5mL