Match the following descriptions to the most appropriate methodof genome engineering presented within the list. Use each answeronce. List: MuGENT, Transformation, CRISPR, Transposonmutagenesis, and MAGE.
a. You are attempting to see the effect of substituting similaramino acids across the genome (called conservative replacement).Thus, by using _____ you try and replace all glycine codons withalanines.
b. You are looking to create a heterogenous mixture of knockoutmutants from a pool of 6 genes.
c. A broadly-applicable technique that can make efficient geneedits based on guide RNA.
d. You are looking to randomly disrupt genes within the genometo assess their function.
e. Using a PCR fragment with homology to the genome, you mustget this DNA into your target cells somehow...
