Explain the process of how a protein is sequenced from start to finish. In your answer,...

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Biology

Explain the process of how a protein is sequenced from start tofinish. In your answer, clearly identify the role of2-mercaptoethanol, dansyl chloride, phenylisothiocyanate, andtrifluoroacetic acid.

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All the mentioned chemicals are used in different chemical methods of protein sequencing Protein sequencing refers to the method of determining the amino acid sequence of all or part of a protein or peptide There are two direct methods of protein sequencing a mass spectrometry and b Edman degradation Mass spectrometry is the most widely used method of amino acid sequencing and identification but Edman degradation remains a valuable tool for identification of Nterminal amino acid of the peptide or protein The usual strategy for determining the amino acid sequence of a protein involves following basic steps Multimeric proteins more than one polypeptide chain are separated into their constituting chains by exposure to low pH high concentration of urea or guanidinium hydrochloride and purified Intrachain SS disulfide crossbridges between cysteine residues in the polypeptide chain are cleaved If these disulfides bonds are present between two different chains then step 2 precedes step1 Each polypeptide See Answer

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