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11 Sequential Action of a Signaling Kinase Cascade Imagine that you have two kinases that...

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11 Sequential Action of a Signaling Kinase Cascade Imagine that you have two kinases that you know work sequentially but you don t know in what order they act For the purposes of this question let s call the kinases PK X and PK Y You are given data and need to figure out in what order the kinases function Does PK X phosphorylate PK Y or vice versa You can determine this from the information given When either kinase is individually completely inactivated cells do not respond to the norma extracellular signal Cells containing a mutant form of PK X that is permanently active gain of function respond even in the absence of an extracellular signal Cells with mutations that contain a loss of function in PK Y AND a gain of function in PK X respond even in the absence of a signal A In the unmutated signaling pathway does PK X activate PK Y or does PK Y activate PK X Explain your reasoning B What outcome would you have predicted for a doubly mutant cell line with a gain of function mutation in PK Y and a loss of function mutation in PK X Explain your reasoning

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