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Discuss the following research techniques 1. DNA ligase 2. Plasmid vector

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Biology

Discuss the following research techniques

1. DNA ligase

2. Plasmid vector

Answer & Explanation Solved by verified expert
4.0 Ratings (785 Votes)
1 DNA ligases catalyze the arrangement of a phosphodiester bond between DNA single strands in the duplex formThe covalent linkage of the 5P gathering of one chain with the nearby 3OH gathering of another is combined with the pyrophosphate hydrolysis of the cofactor ATP or NAD Bacterial DNA ligases eg from E coli B subtilis and S typhimurium utilize the hydrolysis of NAD as their vitality source Conversely ATP is the cofactor for DNA ligases from bacteriophages eg T4 and T7 and eukaryotic cells The covalent joining of polynucleotides catalyzed by the DNA ligase is a vital occasion in DNA repair recombination and most strikingly DNA replication which requires the joining of Okazaki pieces the little incipient ssDNA sections created from the duplicating of the less strand At first looked for and found as a polynucleotide joining or fixing action DNA ligases are available pervasively in every single living being Two of    See Answer
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