Describe three different lead discovery approaches (other than rational)!? And how would you discover a lead by...

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Biology

Describe three different lead discovery approaches(other than rational)!?
And how would you discover a lead by a rationalapproach?

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Answer Drug development is the process of bringing a new pharmaceutical drug to the market once a lead compound has been identified by the process of drug discovery There are different approaches for lead discovery as follows 1 Traditional Library Screening The goal of screening of a library in whole or in part is to discover compounds with modest activity against a target The active compounds discovered through a screen are called hits Targets are normally enzymes or receptors so the term activity refers to an IC50 or EC50 value Compounds are normally stored as a stock solution and to be dispensed as needed by robotic equipment Dimethylsulfoxide DMSO is a preferred solvent The purity of compounds in the library is an important factor Reaction side products should be completely removed from a compound in a library Highthroughput screens involve some type of biochemical test for binding of the molecule of interest to a target The screens are done in 96 384 or 1536well microtiter plates As a library is screened small amounts of each member of the library will be placed in each well and tested for activity If each library member is a single compound a well will contain a single compound or a mixture of compounds See Answer

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