Describe how you would identify genes responsible for transcription in E. coli by isolating mutations in...

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Biology

Describe how you would identify genes responsible fortranscription in E. coli by isolating mutations in theirgenes. Remember that these genes are required for cell viability.Include all the steps in the isolation of the mutants and alsodescribe how you will differentiate the mutants you want from thosein other genes necessary for viability. This includes giving me theexpected result for mutants in the genes you want.

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So heres the answer to your question Let us start with the main part of the question that is the steps that are followed in the isolation of mutants The process of cloning say for a particular DNA segment There are enzymes for the restriction process to happen that is the restriction enzymes After this process there is ligation and also the transformation of a gene This entire process is well described See Answer

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