Data Tables and Post-Lab Assessment

Lab Questions

1.     Transcribethe following DNA sequence:

Hb A:

5’ AGT AAC GGC AGA CTT CTC CTC AGG AGTCAG GTG CAC CAT 3’

3'UCA UUG CCG UCU GAA GAG GAG UCC UCAGUC CAC GUG GUA 5'

2.     Translateyour new RNA sequence using the genetic code. Remember that whendetermining your amino acid sequence, the RNA sequence is read from5’ to 3’.

val- val -his- val- ser -ser- glu-glu- ser-ser- pro- leu -ser

Note: the first amino acid will beMet.

3.     Transcribethe following DNA sequence.

Hb S:

5’ AGT AAC GGC AGA CTT CTC CAC AGG AGTCAG GTG CAC CAT 3’

3`UCA UUG CCG UCU GCC GAG GUG UCC UCAGUC CAC GUG GUA 5`

4.     Translateyour Hb S transcript.

val- val- his- val- ser-ser-val-glu-ser- ser- pro- leu - ser

Note: the first amino acid will beMet.

Post-Lab Questions

1.     How longare each of your peptide chains (how many amino acids arepresent)?

The proteins are made up of multiple units of amino acid. Whenwe talk about peptide

chains they are combination of multiple amino acids. Each of thepeptide chain consists

of many amino acids and the number varies.

2.     Whatdifferences exist between your two transcripts? What about yourtranslated product?

3.     Hb Arepresents a section of the 626 base normal hemoglobin beta mRNAsequence. Hb S is from the sickle cell hemoglobin. Is the mutationfound in sickle cell hemoglobin a point mutation or a frameshiftmutation?

4.     Does thesickle cell mutation result in a missense mutation, silent mutationor nonsense mutation? Why?

A missense mutation is thecause of the disease, sickle cell anemia In a missense mutation, achange in one base pair causes a single amino acid to be changed inthe resulting protein. In sicle cell anaemia there is missencemutation in ?-globin gene where glutamic acid (E/Glu) beingsubstituted by valine (V/Val) at position 6.