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Final            Volumeof             Volumeof              Volume of      Final volume (uL)
[PNPP] mM  0.5mM PNPP(ul)  0.2MTris-HCl(ul)  enzyme (uL)
0.01                                                                          100                       1500
0.02                                                                            100                      1500
0.04                                                                            100                        1500
0.06                                                                           100                           1500
0.08                                                                           100                             1500
0.1                                                                        100                            1500
0.2                                                                             100                              1500
0.3                                                                          100                              1500
0.4                                                                            100                               1500