Fill out table and show all calculations Final             Volume of              Volume of               Volume of       Final volume (uL) [PNPP] mM   0.5mM PNPP(ul)   0.2M Tris-HCl(ul)  ...

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Chemistry

Fill out table and show all calculations

Final            Volumeof             Volumeof              Volume of       Final volume (uL)

[PNPP] mM   0.5mM PNPP(ul)   0.2MTris-HCl(ul)   enzyme (uL)

0.01                                                                          100                       1500

0.02                                                                            100                      1500

0.04                                                                            100                        1500

0.06                                                                           100                           1500

0.08                                                                           100                             1500

0.1                                                                        100                            1500

0.2                                                                             100                              1500

0.3                                                                          100                              1500

0.4                                                                            100                               1500

Answer & Explanation Solved by verified expert
3.8 Ratings (466 Votes)
1 total volume of solution 1500micro L Volume of enzyme 100 micro L Volume of 05mM 001 X 1500 05 30microL so volume of 02 M Tris HCl 150030100 1370 micro L 2 total volume of solution 1500micro L Volume of enzyme 100 micro L Volume of 05mM 002 X 1500 05 60microL so volume of 02 M    See Answer
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