Compare and contrast the BCA and Bradford methods for protein concentration determination. How does each work?...

70.2K

Verified Solution

Question

Chemistry

Compare and contrast the BCA and Bradford methods for proteinconcentration determination. How does each work? Give an example ofsomething that interferes with these methods and explain where eachworks better than the other. Be as specific as possible.

Answer & Explanation Solved by verified expert
4.5 Ratings (942 Votes)
BCA method Bradford method Based on Proteincopper chelation Based on Dyebinding based detection Here the peptide bonds in protein reduce Cu2 ions from the copperII sulfate to Cu The amount of Cu2 reduced is proportional to the amount of protein present in the    See Answer
Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:
  • Unlimited Question Access with detailed Answers
  • Zin AI - 3 Million Words
  • 10 Dall-E 3 Images
  • 20 Plot Generations
  • Conversation with Dialogue Memory
  • No Ads, Ever!
  • Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!
Become a Member

Other questions asked by students