Compare and contrast the BCA and Bradford methods for protein
concentration determination. How does each work?...
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Chemistry
Compare and contrast the BCA and Bradford methods for proteinconcentration determination. How does each work? Give an example ofsomething that interferes with these methods and explain where eachworks better than the other. Be as specific as possible.
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BCA method Bradford method Based on Proteincopper chelation Based on Dyebinding based detection Here the peptide bonds in protein reduce Cu2 ions from the copperII sulfate to Cu The amount of Cu2 reduced is proportional to the amount of protein present in the
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