As you may know, gene transcription begins with the assembly ofthe transcriptional machinery (TFIIA-H proteins) at the corepromoter of genes. Where do you think core promoters are morelikely to be located? Nucleosome DNA or Linker DNA?

Go ahead and formulate a hypothesis on the morelikely location of core promoters of genes in DNA, and defend itwith molecular biology arguments (in other words, why would one orthe other be a preferred location for core promoters, based on whatyou know about nucleosome DNA and linker DNA?).

Then, I want you to imagine that you have access to a standardmolecular genetics lab, and I want you to propose anexperimental test of your hypothesis. You don’t need toget too technical, just a description of how you would design anexperiment that tests your hypothesis, indicating samples,treatments, and the measurements or readouts that that you plan totake. Your imaginary lab has the following equipment:

1)  Antibodies forimmunoprecipitation: these are molecules that can bind toseveral kinds of proteins, and allow you to physically pull themdown (along with anything that is bound to them). In this case,imagine that you have access to antibodies that allow you toseparate any proteins bound to DNA and isolate them along with anyDNA bound to them!!

2)DNA sequencer: a machine that allows you to “read”the sequence of a given DNA sample.

3)Core promoter sequences: Information about what atypical core promoter sequence looks like.

So go ahead and design an experiment using any combination ofone or more of the resources listed above, and do not forget todescribe what results you expect to see, shouldyour hypothesis be correct.

Your submission should be 200 words orfewer,

PLEASE use ONLY the equipment mentioned above.