Analytical Chemistry - Determination of Fe in Vitamin tablet: Iron was reduced by 25 mL 6M HCl...

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Chemistry

Analytical Chemistry - Determination of Fe in Vitamintablet:
Iron was reduced by 25 mL 6M HCl and DI water.
Unknown Iron Sample: 10 mL diluted with DI water in 100 mLvolumetric flask,
Iron Standards: 30 mL of 0.7385 mM
2 mL of hydroquinone solution, 3 mL o-phenanthroline solutionand 10 mL of pH 3.5 buffer added to all flasks.
Blank (100 mL volumetric flasks) flask 1 flask 2 flask 3 flask 4
0 mL Standard
50 mL DI Water
1 mL standard
10 mL Unknown
2 mL standard
10 mL Unknown
5 mL standard
10 mL Unknown
10 mL standard
10 mL Unknown
All flasks were diluted to the 100 mL mark. Spectroscopy wasused to measure the absorbance at 510 nm and values of wavelengthand absorbance for 6 runs are known.
Please explain in detail how to plot a Beer-lambert law from mywavelength and absorbance data in excel. How to plot the absorbancevs. iron concentration added in the standards (in Î¼g) and calculatethe Î¼g of iron in the standards and determine the unknownconcentration from the curve?

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3.6 Ratings (413 Votes)

First input your data in the excel sheet like i have donethen select these two column like thisThen click on the INSERT option and choose XY SCATTER I haveset the arrow on the icon You will get your graph ready Rememberthe quantity will See Answer

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