after transforming E colo with the pGLO plasmid, you incubate the
vials with LB broth for...
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after transforming E colo with the pGLO plasmid, you incubate thevials with LB broth for 30 min first. Explain what may happen ifyou skip this step.
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The heat shock increases the permeability of the cell membrane to DNA The duration of the heat shock is critical in the transformation experiments The standard protocol for pGLO transformation of Escherichia coli is
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