5. You extract chromosomal DNA from 5 cultures of bacteria given to you by another researcher....

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Biology

5. You extract chromosomal DNA from 5 cultures of bacteria givento you by another researcher. You are trying to determine if any ofthe cultures are related to each other, or if they are different.You perform a restriction digest with a restriction enzyme AluI andrun the resulting digested DNA out on a 1% agarose gel. The bandingpatterns in lanes 1 and 5 look the same, but the banding patternsin lanes 2, 3, and 4 look different from the rest. What can youconclude from this information?
6. Suppose you want multiple copies of a gene you havesynthesized. How would you obtain the necessary copies by cloning?By PCR?
7. Why did the use of DNA polymerase from the bacteriumThermus aquaticus allow researchers to add the necessaryreagents to tubes in a preprogrammed heating block?
8. Explain the difference between antigenic shift and antigenicdrift. Using the example of influenza, why is there such concernabout the possible emergence of a particularly virulent strain ofinfluenza?

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Answer 5 that means chromosomal dna at 1 and 5 are of same size and at lane 234 are of same size Answer 6 Yes by performing PCR we can get multiple copies of required DNA Answer 7 Thermus aquaticus has proven to be quite a useful organism in the field of Biotechnology as its enzyme Taq polymerase is harvested for use in polymerase chain reactions PCR The reason Taq polymerase is used in PCR as opposed to other forms of the polymerase enzyme is because Thermus aquaticus form of the enzyme is wellsuited for the repetitive heating involved in PCR and will not denature Taq polymerases See Answer

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