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  3. 2. you are given a pure protein sample to characte

2. You are given a pure protein sample to characterize and provided the following information: Its molar...

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Chemistry

2. You are given a pure protein sample tocharacterize and provided the following information:

Its molar extinction coefficient, ε280, is 0.30 litersmicromole-1 cm-1

Its ΔGo for unfolding is 1.5 kcal/mol at 37o (where RT = 0.59kcal/mole)

A) Using a 0.5 cm pathlength cell, you measure the absorbance at280 nm of a 25- fold dilution of your pure protein in solution andfind A280 = 0.45. What is the original concentration of the proteinbefore dilution?

B) What is the concentration of the unfolded form of the proteinin your sample?

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