10. Mutations in the lacI gene (lacI-) constitutively transcribe the lac operon. Mutations in the operator...

60.1K

Verified Solution

Question

Biology

10. Mutations in the lacI gene (lacI-) constitutively transcribethe lac operon. Mutations in the operator region can also occur,also resulting in the constitutive expression of the lac operon.Using the â€œreagentsâ€ described below, design an experiment to testif a mutant that constitutively expresses the lac operon is mutatedin the lacI gene or in the operator.
X-gal â€“ a colorless chemical that is a substrate forB-galactosidase, when cleaved it produces a blue dye that â€œstainsâ€any cells expressing B-galactosidase.
An Fâ€™ strain of E. coli that contains an F plasmid with afunctional lacI gene
An Fâ€™ strain that contains an F plasmid with a functional operatorsite
The F- mutant strain that constitutively expresses the lacoperon
You can make whatever media you want but must specify what mediayou use in each step.

Answer & Explanation Solved by verified expert

4.3 Ratings (592 Votes)

You need to perform two crosses Mutant F containing wildtype lacI gene Mutant F containing wildtype operator After each cross select Mutants containing F plasmid by antibiotic selection method in plate 1 F1 contains plasmid having wildtype lacI gene See Answer

Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:

Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!

Become a Member