Youâ€™ve identified an antigenic protein unique to multidrug resistant Staphylococcus aureus (MRSA). Youâ€™d like to create a...

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Biology

Youâ€™ve identified an antigenic protein unique to multidrugresistant Staphylococcus aureus (MRSA). Youâ€™d like tocreate a MRSA vaccine based on an in vitro transcribedmRNA. Several answers will be acceptable, sincethere are several technical approaches to carry out the same tasks.Specifically:
(1 pt) What molecular method could you use toclone the gene from S. aureus? (Name a specific approachfrom earlier in the course).
(1 pt) What will be the components of your IVTmRNA itself?
(1 pt) What steps could you take to reduce theimmunogenicity of the IVT mRNA?
(1 pt) What is one way you might deliver theIVT mRNA into a patient?
(1 pt) You find that the S. aureusprotein is poorly expressed in human cells. How could you changeyour
Â Â Â Â Â Â Â Â mRNA sequenceto boost ex

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3.6 Ratings (479 Votes)

To clonethe gene from S aureus PCR based cloning would be a good strategy Using gene specific primers with appropriate restriction sites the gene encoding the anigenic protein can be cloned into plasmid vector compatible with in vitro See Answer

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