Your team has posed the question: Does MAD1 control themetaphase checkpoint? The hypothesis was: If MAD1 gives the signalto progress to anaphase after chromosomes have aligned, then agroup of mutant MAD1 cells will more often have imperfectchromosome numbers after a series of cell divisions compared tonormal cells.You investigated this question by comparing the celldivision of cells that have the normal, or “wildtype†MAD1 gene tothe division of cells with the mutant MAD1 gene. In yourexperiments, you compared the number of “aneuploid†cells(imperfect chromosome numbers) to the number of “euploid†(correctchromosome numbers) in your experimental group of MAD1 mutant cellsand your control group of MAD1 normal cells.
| Percent Aneuploid cells in Sample (incorrectchromosome number) |
| Sample 1 | Sample 2 | Sample 3 | Sample 4 | Average | Standard Deviation |
| Control: Normal MAD1 cells | 7% | 15% | 8% | 13% | 10.75% | 3.86 |
| Experimental: Mutant MAD1 cells | 30% | 25% | 37% | 29% | 30.25% | 4.99 |
Results: Average Percent of AneuploidCells after 24 hrs
Control:Normal MAD1 cells had an average of 10 percent aneuploidcells after 24 hours.
Experimental: Mutatnt MAD1 cells had an average of 30 percentaneuploid cells after 24 hours
Question:
Develop a conclusion based on these results. Is the hypothesissupported or rejected? Explain your reasoning.
