Logo
Home Study Material Contact Search
AI Chat FREE
  1. sciences
  2. biology
  3. your qpcr quantification results show that you hav

Your qPCR quantification results show that you have recovered a total of 30ng in 200µl of...

Free

60.1K

Verified Solution

Question

Biology

Your qPCR quantification results show that you haverecovered a total of 30ng in 200µl of AE buffer.First, calculate the concentration of your DNA solution. Second,calculate the volume of DNA to add to the PCR reaction if the totalamount required is 2ng. please show all the answersteps.

Answer & Explanation Solved by verified expert
3.9 Ratings (653 Votes)

1. Concentration of Dna solution = 30 X 10-9 g / 200 X 10-6 l

                                                    = 0.15 X 10-9 X 106 g/l

                                                    = 0.15 X 10-3 g/l

                                                    = 150 g/l or 150 µg / µl

2. 30 X 10-9 g contains ------------ 200 X 10-6 l

     2 X 10-9 g contains --------------     X liter

X = (2 X 10-9 g X 200 X 10-6 l ) / 30 X 10-9 g

X= 400 X 10-6 / 30l

X= 400 X 10-6 / 30l

X = 13.33 µl required for PCR reaction
Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:
  • Unlimited Question Access with detailed Answers
  • Zin AI - 3 Million Words
  • 10 Dall-E 3 Images
  • 20 Plot Generations
  • Conversation with Dialogue Memory
  • No Ads, Ever!
  • Access to Our Best AI Platform: Zin AI - Your personal assistant for all your inquiries!
Become a Member

Other questions asked by students

Q

Given the following list, how many of the compounds are soluble in water? sodium nitrate ammonium permanganate lithium carbonate iron...

Chemistry
Q

Required information Exercise 5A-2 (Algo) Least-Squares Regression [LO5-11] [The following information applies to the questions displayed below.] Bargain Rental...

Accounting
Q

Covalent Bond Review the Covalent Bonds tutorial Then answer the questions Part L An electrically...

Biology
Q

Find all missing side lengths and angle measures of the figures below Parallelogram Rectangle 71...

Geometry
Q

3 For the given expression tan a 5 points Identify quadrant in which angle is...

Basic Math
Q

A climate scientist wishes to estimate the mean hightemperature in Jarkarta, Indonesia.She selects a random...

Basic Math
Q

Answer the following questions on the F test statistic Complete parts a through d below...

Statistics
Q

60 The terminal velocity for an object dropped near the surface of Earth is the...

Algebra
Q

West Inc. granted 5,000 shares of restricted common stock shares at the beginning of 2020...

Accounting
Q

On December 31, 2023, Jen \& Mink Clothing (J\&M) performed the inventory count and determined...

Accounting
Q

value: 3.00 points Yo Problem 11-4A Prepare a statement of cash flows -Indirect method (LO11-2,...

Accounting
Q

Required information Tableau Dashboard Activity: Interpret and Communicate 12-2(Static)[Problem 12-12; LO12-3] ...

Accounting