Your colleague has isolated a mutant bacterial strain and found the dna 5x10^8 bp in length....

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Biology

Your colleague has isolated a mutant bacterial strain and foundthe dna 5x10^8 bp in length. she has asked you to do restrictionmapping. you start by determining the base composition of thebacterium, and find that the bacteria consists of 24% Thymine and26% Guanine. you plan on using the enzyme NOTI which recognizes thesequence 5'-GCGGCCGC-3'
a) How many fragments would you expect assuming that it occursrandomly throughout the genome
b)your colleague also provided several variants of her strain ofbacteria. You test all of them and found differences in theirrespective banding pattern, in a simple statement, what couldaccount for this?

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The bacteria consists of 24 Thymine and 26 Guanine The basepairing rule exists as per a consequence of stability in terms of hydrogen bond formation Adenine and Guanine are together referred to as purines as they have an imidazole ring attached to their pyrimidine ring Cytosine and Thymine are together referred to as pyrimidines as they do not have any such imidazole ring attached to them A double stranded DNA has just barely enough space within its two strands to exactly accommodate only one purine and one pyrimidine Two of the same type will simply not do as two purines would be too large while two pyrimidines will fall too See Answer

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