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You want to use PCR to amplify a 1Kb exon of the human autosomal gene phenylalanine...

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Biology

You want to use PCR to amplify a 1Kb exon of the human autosomalgene phenylalanine hydroxylase (PH) from the genome of a personsuffering from the disease phenylketonuria, which is due to thisenzyme not functioning.

a) You start with 1 nanogram of genomic DNA in your sample tube.Assuming a human haploid genome is 3 x 109 bp, and eachbp has a mass of 660 g/mole, how many moles of haploid genomes arepresent at the start of your PCR experiment?

b) The PH gene is present in a single copy in the haploidgenome. How many template molecules of the PH gene are present atthe start of your PCR experiment? (Give your answer in molecules,not moles of molecules.)

c) You set your PCR machine to run for 25 cycles to amplify your1 kb fragment. Assume every cycle exactly doubles your desiredtarget DNA. At the end of your 25 cycles, how much amplified DNAhave you created, in terms of number of molecules AND mass ofDNA?

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