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You want to express Erythropoietin (EPO) in mass quantities. EPO is a glycoprotein hormone responsible for...

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Biology

  1. You want to express Erythropoietin (EPO) in mass quantities.EPO is a glycoprotein hormone responsible for the control of RBCsynthesis in the bone marrow. It has invaluable medical usages inanimals and cancer therapy. It is important to note that it hashighly complex glycosylations.
  1. Which expression system would you use to appropriately andefficiently express your gene of interest? Briefly explain thereason for your choice.
  1. b. You implement your gene ofinterest into your designed expression system. However, after yourextraction step, you are not able to obtain the desired protein ofinterest. List one possible reason why this occurred and state howyou would address this problem.
  2. c. You decide to produce yourdesired protein in large batches. You obtain a small sample fromyour bioreactor and observe “clumps” within the cells. What do youthink those could be? Based on this answer, propose a possiblesolution.

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