You suspect there is a protein in blood that has antimicrobial properties. Design an experiment to...

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Medical Sciences

You suspect there is a protein in blood that has antimicrobialproperties. Design an experiment to isolate the protein and testitâ€™s ability to inhibit bacterial growth. How would you determineif it is an antimicrobial peptide or a member of the complementfamily?

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Experiment to isolate the protein It is 2 step process 1 Crude extraction A crude extract from the blood will be obtained using the red blood cells were lysed by addition of 083 ammonium chloride solution to the blood at a ratio of 31 The blood cells were collected by centrifugation 700 g 15 min 4 C resuspended in modified phosphate buffer saline PBSX buffer 137 mM NaCl 27 mM KCl 05 mM MgCl2 81 mM Na2HPO4 15 mM KH2PO4 pH 74 and sonicated to release the neutrophil granules These granules will be collected 27 000 g 40 min 4 C suspended in 10 acetic acid and mixed overnight at 4 C to extract the antimicrobial peptides The solution containing the peptides See Answer

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