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You perform a cell free translation experiment like Nirenberg and Matthaei, but you forget to write...

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Biology

You perform a cell free translation experiment like Nirenbergand Matthaei, but you forget to write down what nucleotides youadded to make the mRNA. You precipitate the translated polypeptidesand measure the relative amount of radiolabeled amino acidsincorporated into them. You get 25% proline, 25% threonine, 12.5%glutamine, 12.5% lysine, 12.5% asparagine, and 12.5% histidine.What nucleotides and in what % did you add to make the mRNA? Pleaseexplain step by step.

Why is the answer 50% C and 50% A?

Answer & Explanation Solved by verified expert
4.3 Ratings (536 Votes)
The codon that codes for the amino acids are as follows a Proline CCU CCA CCG CCC b Threonine ACU ACG ACC ACA c Glutamine CAA CAG d Lysine AAA AAG e Asparagine AAU AAC f Histidine CAUCAC In a cell free extraction system used by Nirenberg and    See Answer
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