You intend to use bacteria to produce ethanol for your spin-off business in Palapye. The bacteria...

80.2K

Verified Solution

Question

Biology

You intend to use bacteria to produce ethanol for your spin-offbusiness in Palapye. The bacteria (strain: Ethanol Blue) you have,is a very poor ethanol producer and is very sensitive to ethanol.However, an industrial strain (strain: Ethanol Red), which has bothan elevated ethanol producing and a high ethanol tolerance trait,is available in your laboratory collections for use as a control.Both bacteria sporulate with relative ease. Note that an elevatedethanol producer is always resistant to elevated amounts ofethanol. a) Isnâ€™t it strange that natural bacteria isolates produceethanol? Explain. [2 marks] b) Briefly describe 2 methods (takingadvantage of their sporulation ability only) you would use toimprove the fermentative capacity of Ethanol Blue. [4 marks] c)State any other methods other than evolutionary engineering andrecombinant DNA technology that can be used to develop the strain.[2 marks] d) Is there a correlation between ethanol production andtolerance? [2 marks] Total [10 marks]

Answer & Explanation Solved by verified expert

4.0 Ratings (411 Votes)

aEthanol fermentation is one of the oldest and most important fermentation processes used in the biotechnology industry Many microorganisms including bacteria and yeasts can produce ethanol as the major fermentation product from carbohydrates bprogress has been made in engineering pentose fermentation capacity into the yeast S cerevisiaeFor that purpose two heterologous pathways for Dxylose utilization have been utilized First the genes encoding Dxylose reductase XR and xylitol dehydrogenase XDH from Scheffersomyces Pichia stipitis have been expressed in S cerevisiae This resulted in Dxylose fermentation but also in significant production See Answer

Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:

Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!

Become a Member