You have purified the peotide hormone that binds to the receptor below. To determine its amino acid...

90.2K

Verified Solution

Question

Biology

Youhave purified the peotide hormone that binds to the receptor below.To determine its amino acid sequence you digested the polypeptidewith trypsin and in a separate reaction you cleaved the polypeptidewith cyanogen bromide. Cleavage with trypsin yielded 5 peptidesthat were sewuences by Edman degradation as shown in the following:
1. Ser-Leu
2. Asp-Val-Arg
3. Val-Met-Glu-Lys
4. Ser-Gln-Met-His-Lys
5. Ile-Phe-Met-Leu-Cys-Arg

the fragments were purified using high-performance liquidchromatography, HPLC, with a hydrophobic C18 column. PREDICT whichfragment eould elute last from the column, providing the chemicalbasis for your prediction

Answer & Explanation Solved by verified expert

4.1 Ratings (574 Votes)

Answer 2AspValArg elute last fromcolumnExplanation1 In hydrophobic column of HPLC hydrophobic peptides get bindand their movement is retarded so that highly hydrophobic peptidewill elute lastly In similar way hydrophilic polar peptide willelute firstly as it will not interact with hydrophobic column andits movement not retarded by hydrophobic interaction According tothis above See Answer

Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:

Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!

Become a Member