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You have ordered a set of enzymes that includes a protease, a DNase, and a RNase....

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Biology

You have ordered a set of enzymes that includes a protease, aDNase, and a RNase. Unfortunately, the company that you orderedthem from failed to label the tubes with the specific enzymes andthey are only labeled A, B and C. In your lab you have two strainsof bacteria that are transformable. Strain Q is prototrophic butsensitive to the antibiotic streptomycin and strain R isauxotrophic for tryptophan but resistant to streptomycin. How mightyou devise an experiment to determine which tube containsDNase?

Answer & Explanation Solved by verified expert
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1 We have been asked to find out which of the tubes are DNAse A B or C They contain DNAse RNAse and Protease We will perform a simple agarose gel assay to determine which is the DNAse In the first step we prepare a selective LB medium containing tryptophan and streptomycin this enables the selective cultivation of    See Answer
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