You have just joined a lab and have obtained a flask with J558L cells. You take...

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Biology

You have just joined a lab and have obtained a flask with J558Lcells. You take an aliquot of the cells, dilute the aliquot withTrypan Blue (1:1), and count the cells using a hemocytometer andobtain the following numbers:
Transparent cells: Blue cells:
Quadrant 1 52 1
Quadrant 2 60 3
Quadrant 3 58 2
Quadrant 4 51 2
Quadrant 5 57 1
1) What is the cell viability?
2) What is the density of the cells (living) in the culture?

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3.8 Ratings (725 Votes)

Answer Trypan blue dye when added to the cells a live cell will pump it out and become transparent Whereas dead cells will take the dye and stain blue Therefore all the transparent cells are live cells whereas blue cells are dead cells 1 Total See Answer

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	Transparent cells:	Blue cells:
Quadrant 1	52	1
Quadrant 2	60	3
Quadrant 3	58	2
Quadrant 4	51	2
Quadrant 5	57	1