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You have a viral stock of an unknown concentration and you want to determine the titer...

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Biology

You have a viral stock of an unknown concentration and you wantto determine the titer of the virus by plaque assay. Your lab matehas VERO cells growing in the lab and you decide to use them for aplaque assay. After infection with a range of dilutions, none ofthe plates showed plaques. Give 2 possible reasons why you did notget any plaques.

You repeat the plaque assay using CHO as the target cells thistime. You infect 3 wells with 0.1 ml of a 10-6 dilution of yourstock. The plates show 105, 95 and 100 plaques. What is the titerof your undiluted viral stock?

Answer & Explanation Solved by verified expert
4.4 Ratings (602 Votes)
During plaque assay of virus on VERO cells no plaques were formed Two possible reasons for nonoccurence of the plaques maybe 1 The virus may not be infecting the vero cells ie Vero    See Answer
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