You have 2 strains of bacteria, strain 1 = trpA- trpB- trpC+ and strain 2 =...

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You have 2 strains of bacteria, strain 1 = trpA- trpB- trpC+ andstrain 2 = trpA+ trpB+trpC-. You grow P1 transducing phage onstrain 1 (you infect strain 1 with the phage), then infect strain 2with this lysate and select for transductants on minimal medium(trpA,B,C mutants are all Trp auxotrophs). You get 1000transductants growing with 0.1 ml lysate. You then infect strain 2with your phage, then transduce strain 1 and get 15 transductants.a. What is the order of trpA, trpB, trpC? b. What is the geneticdistance between trpA and trpC?

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Order of trpA trpB trpC Celicolor is isolated into bunches We have appeared right now the trpC trpB and trpA qualities are situated on one bunch with a novel short ORF trpX situated between the trpC and trpB qualities while trpD structures a different locus a huge good ways from the trpCXBA group Haloferax volcanii Mycobacterium tuberculosis 9 and streptomycetes are special in having the quality request trpCtrpBtrpA which is not the same as the requests trpCtrpFtrpBtrpA or trpFtrpBtrpA found in other eubacteria and archaebacteria 10 Shockingly Corynebacterium glutamicum some time ago Brevibacterium lactofermentum the high G C proportion Grampositive bacterium other than mycobacteria and streptomycetes for which the quality request is known has the more typical eubacterial quality request The similitude of quality structure among mycobacteria and streptomycetes is likewise reflected in the degree of character in addition to the likeness of the Trp proteins See Answer

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