You determined in the previous assignment the exact volume of 50X TAE that you will need....

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Biology

You determined in the previous assignment the exact volume of50X TAE that you will need. I suggest that you make 40 ml of 50XTAE so that you have some extra (just in case you need it). Therecipe for one liter of 50X TAE is as follows: Â¥ 242g Tris base Â¥57.1 ml glacial acetic acid Â¥ 100 ml 0.5 M EDTA pH 8.0 9. Provide arecipe for 40 ml of 50X TAE. 10. When you make your agarose gel,you will need to make 50 ml of 1% (w/v) agarose in 1X TAE. How muchagarose will you require? [you will make this later, not onSolution day]

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4.3 Ratings (931 Votes)

Hi Answer The below given calculations are the simplest calculations to understand how the volume reduces with change in concentration so try to understand step by step As the recipe of 1 litre 50X TAE is given in question ie 50X TAE 1 L 242 g Tris base 571 mL Glacial acetic acid 100 mL 05 See Answer

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