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You decide to use RT-PCR to confirm your predictions about the exon organization of the 2...

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Biology

You decide to use RT-PCR to confirm your predictionsabout the exon organization of the 2 mRNAs in tissue such as heartand epidermis. Describe in general the experiment you would do,specifying where your PCR primers would be designed to bind. Whatresults would you expect. Please be specific

Answer & Explanation Solved by verified expert
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RT PCR is also referred to as reverse transcription polymerase PCR This is the most sensitive form of PCR in which there would be detection of mRNA and there would be quantification of this detected rna The samples used in this technique are much more minute as compared to the othe techniques There are two protocols for this method one is one step and the second is 2 step process In the one step RT PCR there would be mRNA targets which have a    See Answer
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