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You decide to dilute a broth culture by making a series of four 1:100 dilutions. You...

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Biology

You decide to dilute a broth culture by making a series of four1:100 dilutions. You then pipet 1 ml of the final dilution onto oneagar plate and 0.1 ml onto a second plate. After incubatingovernight, you count 350 colonies on the first plate (with 1 ml)and 56 colonies on the second plate (with 0.1 ml). Which platewould you use for your calculations? Why? Calculate theconcentration of the original culture and be sure to include theproper units in your answer. (Hint: Draw a diagram of the dilutionseries to help you get the answer.)

Answer & Explanation Solved by verified expert
4.1 Ratings (465 Votes)
I will count the second plate with 56 colonies fornearaccurate calculations because1 Counting 350 colonies on the agar plate is tedious prone tomistakes and differentiate between    See Answer
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