You are studying the part of the brain that mediates stress. You have treated some mice...

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Biology

You are studying the part of the brain that mediates stress. Youhave treated some mice under stressed conditions and you also havecontrol mice. You isolate the appropriate brain regions from bothgroups. Describe what you would do to identify changes in genesassociated with acetylated lysine 9 on histone H3. What would youdo to prepare the brain samples for analysis? Would technique wouldyou use to purify genes associated with aceylated lysine 9 histoneH3 and how does it work? How would you identify all the genes inthe purified samples? What kind of data would you get in the endthat would tell you what genes changed in their association withacetylated lysine 9 histone H3 in response to stress?

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3.8 Ratings (664 Votes)
Here to perform this study we need both biochemical and global gene expression analysis First we need to grow mice in stressed and control conditions After the stress has been applied we need to isolate the brain region of interest in both experimented and control mice We need to perform a    See Answer
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