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You are studying Drosophila melanogaster and you hypothesize that a particular protein called “Eyeless” is essential for...

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Biology

  1. You are studying Drosophila melanogaster and youhypothesize that a particular protein called “Eyeless” is essentialfor the eye to form.

  1. You first decide to use the Gal4-UAS system to makesure that Eyeless is expressed in the eye. To do this you havefound what you believe is the eye-specific enhancer for theEyeless gene. You also decide to use GFP as a readout forprotein production induced by the Eyeless enhancer.Describe how you will use the Gal4-UAS system and thesecomponents to determine if the Eyeless proteins might normally beexpressed in the eye. Be sure to make it clear how the system worksand how you have set it up. (12 points)
  1. To determine if the Eyeless protein is required for the eye toform, you need to test what would happen if the fly developswithout it! How could you use the Gal4-UAS system slightlydifferently to specifically inhibit thetranslation of the Eyeless mRNA in theeye to test this? (6 points)

Answer & Explanation Solved by verified expert
3.7 Ratings (408 Votes)
Ans The Gal4UAS system is one of the most powerful tools for thestudy of cellular and developmental processes inDrosophila Gal4 drivers can be used to induce targetedexpression of dominantnegative and dominantactive proteinshistological markers activity sensors genespecific dsRNAsmodulators of cell survival or proliferation and other reagentsDemonstrate the induction of predicted    See Answer
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