You are studying a bacterium that grows in a particularecological niche. You cannot culture it in the laboratory, but youcan isolate small quantities of cells that microscopic analysisindicates are not contaminated with other bacteria. How will youobtain the ribosomal RNA gene sequence data to study the taxonomyof the bacterium? •How will you determine the complete genomesequence of the bacterium?

The steps performed for determining the complete genomicssequence of the bacterium include- Step 1: Cloning Step 2:Restriction Mapping Step 3: Gel Electrophoresis Step 4: DNASequence Analysis

Gel electrophoresis- The DNA fragments after Restrictiondigestion are separated by electrophoresis, a process that involvesapplication of an electric field to cause the DNA fragments tomigrate into an agarose gel. The gel is then stained with amethylene blue stain to visualize the DNA bands and may bephotographed. Although methylene blue dye is not as sensitive asethidium bromide it may be used to stain the higher quantities ofDNA that are used in this experiment. The movement of the fragmentswill always be towards the positive electrode because DNA is anegatively charged molecule. The fragments move through the gel ata rate that is determined by their size and shape, with thesmallest moving the fastest.

DNA Sequence Analysis- This method uses dideoxynucleotidetriphosphates(ddNTPs) whichwhich an H on the 3’ carbon of theribose sugar instead of the normal OH found in deoxynucleotidetriphosphates (dNTPs). Dideoxynucleotides are chain terminators. Ina synthesis reaction, if a dideoxynucleotide is added instead ofthe normal deoxynucleotide, the synthesis stops at that pointbecause the 3’OH necessary for thetaddition of the next nucleotideis absent. The sequencing gel is able to resolve fragments thatdiffer in size from each other by only one base.

**I need explain exactly gel ectrphoresis and DNAsequence analysis will help find the bacteria genome