You are studying a bacterium that grows in a particular ecological niche. You cannot culture it...

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Biology

You are studying a bacterium that grows in a particularecological niche. You cannot culture it in the laboratory, but youcan isolate small quantities of cells that microscopic analysisindicates are not contaminated with other bacteria. How will youobtain the ribosomal RNA gene sequence data to study the taxonomyof the bacterium?
â€¢How will you determine the complete genome sequence of thebacterium?
the steps should be:
Step 1: Cloning Step 2: Restriction Mapping Step 3: GelElectrophoresis Step 4: DNA SequenceAnalysisÂ Â gel electrophoresis and dna analysisneeds to elaborated on.

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4.3 Ratings (743 Votes)

PCR with primers to regions of ribosomal RNA genes that are conserved across a wide range of bacteria to amplify the corresponding sequences from the bacterium of interest could be used If it appeared to be a single band in gel electrophoresis ie the PCR product is sufficiently specific and was in enough quantity then the DNA sequence could be determined directly If the product is not specific the the PCR might need See Answer

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