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You are removing imidazole from your pooled nickel column samples by dialysis. The volume of the pooled...

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Question

Chemistry

You are removing imidazole from your pooled nickel columnsamples by dialysis. The
volume of the pooled sample is 32 mL and the imidazoleconcentration is 0.5 M.

a. What is the concentration of imidazole after exposure tothree consecutive dialyses
against 1 L of buffer?
b. If the imidazole at the start is 3.4% (w/v), what is theconcentration in
weight percent at the end of the dialysis (three consecutiveexposures to one liter
of buffer)?

Answer & Explanation Solved by verified expert
4.0 Ratings (454 Votes)
a Volume of sample V132ml Strength of imadazole initially S105M Volume after 1st dialysis1000ml 32 ml volume of buffer sampleV2 Strength of imadazole after 1st dialysisS2 Therefore S1V1S2V2 32ml 05M 1032mlS200155 M    See Answer
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